Iranian Scorpions from Two Families–
Iranian scorpions consist of two scurry and buddy families. Scorpio Mesobutos Eposus is a bestial family dispersed in different parts of the country.
Regarding the general dispersion of the scorpion of Iran, recalling this point, it is necessary that the radius of the gloomy geographical dispersion in Iran is greater.
The dwarf scurry is limited to parts of the south, southwest, central and Azeri provinces, Kermanshah, Kurdistan and Khorasan,
While various types of dark-haired genus have been reported from all parts of Iran.
The wide variation and dispersion of the globular dark matter in Iran can be due to its more ancient history than the scrubbing dark. Considering that Iran’s Mesopotamus scrotum is deadly, isolating and studying the poisonous components of this scorpion venom can provide an antidote to provide better and more effective medical services to scorpion people and provide an appropriate response to death. Mir and severe complications from this type of scorpion will be. Recently, the effect of this poison on the respiratory and cardiac system was investigated at the Razi serum center.
The major effect of toxins is on the blockage of sodium, potassium and chloride channels. Toxins with a chain larger than 60 amino acids more affect sodium channels and 30 to 40 amino acid chains on potassium and chloride channels. To purify the toxin, the electrical stimulation of the glandular gland is also used.
All scorpions have a venomous sting, but the vast majority of the species do not represent a serious threat to humans, and in most cases, healthy adults do not need any medical treatment after being stung. Only about 25 species are known to have venom capable of killing a human.1 In some parts of the world with highly venomous species, human fatalities regularly occur, primarily in areas with limited access to medical treatmen
Methods of tracing of scorpions-
Methods of extraction of scorpion venom are different, the two main methods are electric shock and endodontic. Experience has shown that the first method is more suitable and large proteins such as mucoe are less secreted in the venom.
Electric shock method-
To do this, the live scorpion with a pair of pins from the head and tail of the head and tail tuck the animal into a small glass dish, placing two electrodes of the ectopic device for a moment on the sides of the gland.
Due to the action of the contraction of the dependent muscle, pure poison is drained inside the container. The primary secretions of the poison are clear and clear, but gradually becomes cloudy and sticky. In this case, the toxin is mixed with the mucus.
Endodontic method-In this method, they use the tail end of the scorpion, which is called a poison gland. (The tail of the scorpions contains 5 clauses)
For poisoning, first cut off the toxic gland from the attachment to the last tail (fifth paragraph) and rinse it, then place it in the drying apparatus for about one month to dry completely, then the tubers Crushed into crystalline mortars to be crushed, then dissolved with distilled water. The solution is placed in a centrifuge to separate the contents of the poison.Suitable voltage for scorpion poisoning-
The amount of voltage and frequency required for electrical stimulation varies depending on scorpion type. Typically, voltages of 6 to 10 volts can produce clear poisons. At higher voltages, the possibility of mucus secretion increases.
Maintain the resulting poison-
The toxins obtained from either of these two methods can be dried and vacuumed in a dry state and in powder form. The distiller can also be used to dry the toxin solution. In this case, the dried poison is crystallized.
The toxins are stored in sealed glass and in a dry and dark place. 2 to 4 degrees Celsius is a good temperature for storing poison. Trapping should be done from the species and, if possible, from the same subspecies, all belonging to a population and a region.
Sam Scorpion is a protein substance that is fresh and purified, clear and colorless, with a neutral to alkaline pH. Its white powder has a bright cream and its crystal is yellow.
The broadest technique used to purify scorpion venom toxins involves extraction of crude poison solution during water dissolution and centrifugation, followed by column chromatography. Toxins are play polypeptides, so a cathodine replacement resin such as carboxymethyl cellulose seems appropriate for the second stage of purification, but usually a third stage of chromatography is needed to get the toxins in the form of a homogeneous form.
